Proprietary R&D

In-house R&D on development of novel platform technologies and drug discovery

Advanced gene cloning tools

Conventional DNA engineering tools often present a major bottleneck for the highly complex demands of modern “synthetic biology” and metabolic engineering approaches.

ACIES BIO has developed a rapid one-step cloning and gene manipulation tools platform by combining large accommodation capacity of artificial chromosome vectors (YAC), TAR-assisted cloning and recombineering techniques, for rapid manipulation of large gene clusters in S. cerevisiae and their efficient transfer to the industrial heterologous hosts. We achieved this by constructing specific bifunctional “Large Pathway Cloning Vectors” (LPCVs), which enable selective in vivo cloning of desired large DNA fragments of interest in yeast. In the next step, LPCV is isolated from yeast and transformed to E. coli, and finally delivered into final heterologous host as prominent secondary metabolite producer (e.g. a high-producing Actinomyces strain).

These in-house developed LPCV vectors allow us to:

  • clone and heterologously express very large DNA segments (over 100kb)
  • “genome-mining” for new complex biosynthetic pathways and secondary metabolites
  • quickly transfer entire biosynthetic pathway encoding known (commercial) metabolite from a wild-type or a poor-growing strain into a high-producing industrial strain

When necessary for efficient expression, state-of-the-art synthetic biology approaches are also being used by ACIES BIO, including re-factoring or de-novo synthesis of the entire biosynthetic gene clusters.
ACIES BIO advanced gene tools platform allows our clients to quickly obtain the target biosynthetic gene cluster of interest for efficient manipulation and/or heterologous expression for production of highly valuable bioactive metabolites from difficult-to cultivate organisms.

Based on the above mentioned success stories, ACIES BIO has set up a novel ABCloneTM cloning service, which enables you to have the desired DNA fragment quickly cloned into a plasmid of your choice, avoiding time-consuming and cost-intensive research. For more information, click here.

Scientists at ACIES BIO made a breakthrough innovation: high-value technology that uses unique and economic bioprocess to convert acid waste whey from dairy factories into high value animal feed products (vitamin B12) and clean water in the same process. Check out Whey2Value!

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ABCloneTM is fast and efficient solution for cloning of large natural and synthetic DNA fragments. We provide ABCloneTM as a stand alone service for getting quickly your long DNA fragment sub-cloned into plasmid vector of your choice.

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European Medicines Agency (EMA) granted orphan medicinal product designation to its lead compound CAB1803 for Pantothenate-kinase-associated neurodegeneration (PKAN)

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Acies Bio received the “Jesenko Award” from Biotechnical Faculty of University of Ljubljana. The award recognizes institutions for their outstanding research work in the field of biotechnology.

Within H2020 SME Innovation Associate project we invite a “Disease Model Development Manager” to join our team.

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