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Advanced gene cloning

ABClone: Fast and efficient solution for cloning of large natural and synthetic DNA fragments

ABCloneTM is an advanced cloning service, which provides a fast and efficient route to obtaining large DNA fragments, ranging from 20 kbp to several 100 kbp and even upwards of 1 Mbp in size. ABCloneTM is based on one-step highly accurate in vivo recombinational cloning technology, mastered at ACIES BIO through decades of experience in molecular biology. We can adapt ABCloneTM to address specific requirements of your project by: 

ABCloneTM delivers error-free DNA fragments and is routinely used for cloning of large and extremely complex gene clusters encoding biosynthesis of bacterial secondary metabolites. Some recent examples are several over 100 kbp long biosynthetic clusters from Actinomycetes, encoding biosynthesis of polyketide antibiotics, large biosynthetic gene clusters from Burkholderiales and other exotic microorganisms, for as well as human immunoglobulin genes. Importantly, cloning DNA fragments directly from genomic DNA by ABCloneTM avoids the risk of introducing sequence errors frequently occurring in published genomic sequences.

The ABCloneTM service could be customized to meet your specific requirements, including providing a detailed project design, prepared by highly experienced molecular biologists, and delivery of progress report from dedicated project manager to keep you informed.

Use the ABCloneTM as stand alone cloning service or have ACIES BIO introduce your fragment into a target microbial strain and assess the expression / productivity in the scope of a full technology development project. With years of experience in industrial strain improvement, bioprocess development, natural product biosynthesis and downstream / isolation procedures ACIES BIO is dedicated to make every project a success!

Please check our flyer for details:  ABClone Flyer.pdf

Send your DNA sequence(s) and cloning vector specifications to #EM#6163616f6b6b6347696a636e7f6f67603e727d7e#EM# and receive a quote within days.

Acies Bio received the “Jesenko Award” from Biotechnical Faculty of University of Ljubljana. The award recognizes institutions for their outstanding research work in the field of biotechnology.

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ABCloneTM is fast and efficient solution for cloning of large natural and synthetic DNA fragments. We provide ABCloneTM as a stand alone service for getting quickly your long DNA fragment sub-cloned into plasmid vector of your choice.

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