STRAIN DEVELOPMENT
Strain engineering
At Acies Bio, we stand at the forefront of strain engineering, offering cutting-edge genetic/metabolic engineering know-how. Backed by an array of genetic elements and state-of-the-art genome editing tools (e.g., CRISPR), we have developed a diverse set of strains. We are ready to quickly bring new biosynthesis pathways into an appropriate production strain for a vast variety of biomolecules. With almost 20 years of experience in genetic engineering of multiple microbial hosts we can choose the optimal starting strain. We work with classical laboratory workhorses E. coli or S. cerevisiae, different strains of yeasts (e.g. Yarrowia lipolytica, Pichia pastoris), different bacilli strains, filamentous bacteria, fungi, and others. For each class of molecules, we select the most appropriate chassis strain and, using synthetic biology or metabolic engineering approaches, quickly demonstrate PoC for the target molecule. This is followed by technology optimization and scale-up, aiming to rapidly showcase the performance of the technology in a real industrial environment.
High-throughput strain evolution
Through a non-GM high-throughput (HTP) strain evolution, we can enhance existing traits of microbial production strains, such as the production of target molecules, reduction of by-products or impurities, and adaptation of growth rate in non-standard and defined media. Our SmartRouteTM innovation extends beyond traditional mutagenesis methods coupled with HTP screening to include rational pre-screening using antimetabolites or toxic inhibitors and enrichment of improved mutants with advanced automated adaptive laboratory evolution (ALE) and microfluidics systems, ensuring the HTP screening efficiency and increasing frequency of mutants with positive trait improvements. When evolving a strain, we do not rely solely on the screening capacity but also on the individualized development of robust and scalable screening assays, merging strain evolution expertise with our profound knowledge of microbiology and bioprocess development.
Our SmartRouteTM to strain improvement or strain engineering is driven by ourscreening assay development, mutagenesis expertise, microfluidic systems, scale-upand demonstration of improvement at bioreactor scale, and powerful analytical support, offering a completepackage for strain improvement.
novel antibacterial polyketide by Myxobacteriareduction of impurities in an antibiotichigh yield strain for a vitaminheterologous polyketide expressionindustrial protein productionindustrial API technology
rapid non-GMO strain improvement in 6 months
increase of yield from 20 mg/L to 500 mg/L
25 g of previously unavailable API delivered
GMO without residual antibiotic markers
80% reduction of key impurity
no loss of target compound yield
metabolic engineering
about 30 copies of gene operon amplified in the genome
yield increased from 0.1 g/L to over 25 g/L
60 kb gene cluster cloned
heterologous polyketide production achieved
proprietary heterologous high expression platform used
secretion mechanism used; 90% secreted
protein tagged to simplify DSP
start with a wt-strain
24 months
from 100 mg/L to 10 g/L