State-of-the-art R&D support from conceptualization, research to product development

Strain improvement

Industrial strain development and strain improvement programs

2 1   strain
ACIES BIO provides an efficient high-throughput strain improvement program, which can significantly increase the yield of the client’s target product or improve the impurity profile of the target compounds. ACIES BIO generally uses two approaches to strain improvement: (a) smart random mutagenesis and high-throughput strain selection using a combination of classical and so called “rational mutagenesis” as well as genome shuffling / protoplast fusion approaches, and (b) biosynthetic / metabolic engineering of identified target pathways, using over-expression of target genes and clusters and/or inactivation of competing or undesired metabolic pathways

Acies Bio is proficient in industrial application of “omics” (genomics / transcriptomics / proteomics) approaches in identification of key metabolic pathways involved in the biosynthesis of target products or undesired impurities. 
Classical Approach – strain selection

  • classical mutagenesis and high-throughput strain selection and analytics
  • directed mutagenesis by metabolic pressure
  • scale-down process optimization
  • starting from existing technologies or from wild type strains

Genetic Approach – metabolic engineering

  • increasing precursor supply
  • up / down-regulation of genes and/or pathways
  • removing competing metabolic pathways and impurities

Our strain improvement projects are generally supported by genome analysis and bioinformatics support, to identify the metabolic potential and existing bottlenecks of the strain, which can be essential when designing research strategies for efficient yield increase. Whole-genome analysis also provides additional security in strain development as well as in an eventual IP support.

Decades of experience in industrial strain improvement programs and routine manipulation of a number of different bacterial species (E.coli, Bacillus, actinomycetes, myxobacteria, yeasts, etc.) using in-house developed molecular biology tools and techniques allow us to efficiently engineer and improve target strains at a very high success rate and rapid development time-frames.

Although each strain improvement / strain development project is unique, typically the key elements of our strain improvement program would include:

  • whole-genome sequencing and bioinformatics analysis of the starting strain
  • scaling-down the bioprocess to obtain reproducible high-throughput screening at smallest possible scale
  • classical and directed mutagenesis combined with high-throughput screening
  • genetic / metabolic engineering (if GMO acceptable for the client)
  • optimization and stability testing of improved candidate strains
  • potential media and process re-optimization
  • scale-up to high-volume fermentation

Acies Bio received the “Jesenko Award” from Biotechnical Faculty of University of Ljubljana. The award recognizes institutions for their outstanding research work in the field of biotechnology.

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