SmartRoute to protein engineering

27.05.2025

In today's biotechnology landscape, AI and machine learning have revolutionized enzyme engineering, enabling rapid generation of thousands of promising variants. However, this creates a critical bottleneck: how to efficiently validate and scale these variants under real-world conditions?

Based on its SmartRoute platform Acies Bio developed a seamless workflow from variant screening to industrial production, combining cutting-edge technology with 18 years of biotechnology expertise.

Correlation of enzymatic activity of three protease variants in ultra high-throughput microfluidic assay and classical high-throughput screening in microtiter plates.

High-Throughput Screening with SmartFluidics

- Screen hundreds of thousands of variants daily using our advanced droplet microfluidics platform

- Seamlessly integrate with NGS sequencing for iterative AI optimization across multiple generations

- Test enzymes expressed in actual industrial production strains, not just laboratory systems

- Use both commercial and custom-designed substrates for maximum flexibility

- Pre-filter vast libraries before moving to complex physiological testing

Comprehensive Validation

- Evaluate promising candidates in both cell-free systems and complex metabolic pathways

- Industry-standard analytics: HPLC, LC-MS, and spectroscopic techniques

- Structured data collection enabling seamless integration with AI workflows

- Testing under real industrial conditions

Industrial Scale-Up and Production

- Proprietary high-yielding expression platforms that can be rapidly adapted to your needs

- Robust expression systems in key industrial organisms (Bacillus, Pichia pastoris, E. coli)

- In-house fermentation development (5-100L)

- Full-scale production capability up to 10,000L

- Complete downstream processing and purification

- Kilogram-scale protein production

- Long-term supply partnership options

Your One-Stop Partner

From screening AI-generated variants to delivering industrial quantities of optimized enzymes, Acies Bio provides the expertise and infrastructure to transform computational innovations into commercial success. Our integrated approach eliminates handoff delays and scaling challenges, accelerating your path to market.